Articles | Volume 10, issue 2
https://doi.org/10.5194/soil-10-521-2024
https://doi.org/10.5194/soil-10-521-2024
Original research article
 | 
19 Jul 2024
Original research article |  | 19 Jul 2024

Improving measurements of microbial growth, death, and turnover by accounting for extracellular DNA in soils

Jörg Schnecker, Theresa Böckle, Julia Horak, Victoria Martin, Taru Sandén, and Heide Spiegel

Related authors

Microbial activity responses to water stress in agricultural soils from simple and complex crop rotations
Jörg Schnecker, D. Boone Meeden, Francisco Calderon, Michel Cavigelli, R. Michael Lehman, Lisa K. Tiemann, and A. Stuart Grandy
SOIL, 7, 547–561, https://doi.org/10.5194/soil-7-547-2021,https://doi.org/10.5194/soil-7-547-2021, 2021
Short summary

Cited articles

Ascher, J., Ceccherini, M. T., Pantani, O. L., Agnelli, A., Borgogni, F., Guerri, G., Nannipieri, P., and Pietramellara, G.: Sequential extraction and genetic fingerprinting of a forest soil metagenome, Appl. Soil Ecol., 42, 176–181, https://doi.org/10.1016/j.apsoil.2009.03.005, 2009. 
Bååth, E.: Estimation of fungal growth rates in soil using 14C-acetate incorporation into ergosterol, Soil Biol. Biochem., 33, 2011–2018, https://doi.org/10.1016/S0038-0717(01)00137-7, 2001. 
Birgander, J., Olsson, P. A., and Rousk, J.: The responses of microbial temperature relationships to seasonal change and winter warming in a temperate grassland, Glob. Change Biol., 24, 3357–3367, https://doi.org/10.1111/gcb.14060, 2018. 
Blazewicz, S. J. and Schwartz, E.: Dynamics of 18O Incorporation from H2 18O into Soil Microbial DNA, Microb. Ecol., 61, 911–916, https://doi.org/10.1007/s00248-011-9826-7, 2011. 
Brookes, P. C., Landman, A., Pruden, G., and Jenkinson, D. S.: Chloroform fumigation and the release of soil nitrogen: A rapid direct extraction method to measure microbial biomass nitrogen in soil, Soil Biol. Biochem., 17, 837–842, https://doi.org/10.1016/0038-0717(85)90144-0, 1985. 
Download
Short summary
Microbial processes are driving the formation and decomposition of soil organic matter. In contrast to respiration and growth, microbial death rates currently lack distinct methods to be determined. Here, we propose a new approach to measure microbial death rates. This new approach to determine microbial death rates as well as dynamics of intracellular and extracellular DNA separately will help to improve concepts and models of C dynamics in soils in the future.
Share