Articles | Volume 6, issue 2
https://doi.org/10.5194/soil-6-399-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/soil-6-399-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Short- and long-term temperature responses of soil denitrifier net N2O efflux rates, inter-profile N2O dynamics, and microbial genetic potentials
Kate M. Buckeridge
CORRESPONDING AUTHOR
Department of Ecology and Evolutionary Biology and Kansas Biological Survey, University of Kansas, Lawrence, KS, USA
now at: Global Academy of Agriculture and Food Security, The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK
Kate A. Edwards
Natural Resources Canada, Canadian Forest Service, Ottawa, ON, Canada
Kyungjin Min
Department of Ecology and Evolutionary Biology and Kansas Biological Survey, University of Kansas, Lawrence, KS, USA
now at: Department of Life and Environmental Sciences, University of California-Merced, Merced, CA, USA
Susan E. Ziegler
Department of Earth Sciences, Memorial University, St. John's, NL, Canada
Sharon A. Billings
Department of Ecology and Evolutionary Biology and Kansas Biological Survey, University of Kansas, Lawrence, KS, USA
Related authors
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Alessia A. Colussi, Daniel Persaud, Melodie Lao, Bryan K. Place, Rachel F. Hems, Susan E. Ziegler, Kate A. Edwards, Cora J. Young, and Trevor C. VandenBoer
Atmos. Meas. Tech., 17, 3697–3718, https://doi.org/10.5194/amt-17-3697-2024, https://doi.org/10.5194/amt-17-3697-2024, 2024
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A new modular and affordable instrument was developed to automatically collect wet deposition continuously with an off-grid solar top-up power package. Monthly collections were performed across the Newfoundland and Labrador Boreal Ecosystem Latitudinal Transect of experimental forest sites from 2015 to 2016. The proof-of-concept systems were validated with baseline measurements of pH and conductivity and then applied to dissolved organic carbon as an analyte of emerging biogeochemical interest.
Keri L. Bowering, Kate A. Edwards, and Susan E. Ziegler
Biogeosciences, 20, 2189–2206, https://doi.org/10.5194/bg-20-2189-2023, https://doi.org/10.5194/bg-20-2189-2023, 2023
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Dissolved organic matter (DOM) mobilized from surface soils is a source of carbon (C) for deeper mineral horizons but also a mechanism of C loss. Composition of DOM mobilized in boreal forests varied more by season than as a result of forest harvesting. Results suggest reduced snowmelt and increased fall precipitation enhance DOM properties promoting mineral soil C stores. These findings, coupled with hydrology, can inform on soil C fate and boreal forest C balance in response to climate change.
Allison N. Myers-Pigg, Karl Kaiser, Ronald Benner, and Susan E. Ziegler
Biogeosciences, 20, 489–503, https://doi.org/10.5194/bg-20-489-2023, https://doi.org/10.5194/bg-20-489-2023, 2023
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Boreal forests, historically a global sink for atmospheric CO2, store carbon in vast soil reservoirs. To predict how such stores will respond to climate warming we need to understand climate–ecosystem feedbacks. We find boreal forest soil carbon stores are maintained through enhanced nitrogen cycling with climate warming, providing direct evidence for a key feedback. Further application of the approach demonstrated here will improve our understanding of the limits of climate–ecosystem feedbacks.
Frances A. Podrebarac, Sharon A. Billings, Kate A. Edwards, Jérôme Laganière, Matthew J. Norwood, and Susan E. Ziegler
Biogeosciences, 18, 4755–4772, https://doi.org/10.5194/bg-18-4755-2021, https://doi.org/10.5194/bg-18-4755-2021, 2021
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Soil respiration is a large and temperature-responsive flux in the global carbon cycle. We found increases in microbial use of easy to degrade substrates enhanced the temperature response of respiration in soils layered as they are in situ. This enhanced response is consistent with soil composition differences in warm relative to cold climate forests. These results highlight the importance of the intact nature of soils rarely studied in regulating responses of CO2 fluxes to changing temperature.
William R. Wieder, Derek Pierson, Stevan Earl, Kate Lajtha, Sara G. Baer, Ford Ballantyne, Asmeret Asefaw Berhe, Sharon A. Billings, Laurel M. Brigham, Stephany S. Chacon, Jennifer Fraterrigo, Serita D. Frey, Katerina Georgiou, Marie-Anne de Graaff, A. Stuart Grandy, Melannie D. Hartman, Sarah E. Hobbie, Chris Johnson, Jason Kaye, Emily Kyker-Snowman, Marcy E. Litvak, Michelle C. Mack, Avni Malhotra, Jessica A. M. Moore, Knute Nadelhoffer, Craig Rasmussen, Whendee L. Silver, Benjamin N. Sulman, Xanthe Walker, and Samantha Weintraub
Earth Syst. Sci. Data, 13, 1843–1854, https://doi.org/10.5194/essd-13-1843-2021, https://doi.org/10.5194/essd-13-1843-2021, 2021
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Data collected from research networks present opportunities to test theories and develop models about factors responsible for the long-term persistence and vulnerability of soil organic matter (SOM). Here we present the SOils DAta Harmonization database (SoDaH), a flexible database designed to harmonize diverse SOM datasets from multiple research networks.
Keri L. Bowering, Kate A. Edwards, Karen Prestegaard, Xinbiao Zhu, and Susan E. Ziegler
Biogeosciences, 17, 581–595, https://doi.org/10.5194/bg-17-581-2020, https://doi.org/10.5194/bg-17-581-2020, 2020
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We examined the effects of season and tree harvesting on the flow of water and the organic carbon (OC) it carries from boreal forest soils. We found that more OC was lost from the harvested forest because more precipitation reached the soil surface but that during periods of flushing in autumn and snowmelt a limit on the amount of water-extractable OC is reached. These results contribute to an increased understanding of carbon loss from boreal forest soils.
Michael Philben, Sara Butler, Sharon A. Billings, Ronald Benner, Kate A. Edwards, and Susan E. Ziegler
Biogeosciences, 15, 6731–6746, https://doi.org/10.5194/bg-15-6731-2018, https://doi.org/10.5194/bg-15-6731-2018, 2018
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We explored the relationship between chemical composition and the temperature sensitivity of moss decomposition using 959-day lab incubations. Mass loss was low despite the predominance of carbohydrates, indicating the persistence of labile C. Scanning electron microscopy revealed little change in the moss cell-wall structure. These results suggest that the moss cell-wall matrix protects labile C from decomposition, contributing to the globally important stocks of moss-derived C.
Bryan K. Place, Aleya T. Quilty, Robert A. Di Lorenzo, Susan E. Ziegler, and Trevor C. VandenBoer
Atmos. Meas. Tech., 10, 1061–1078, https://doi.org/10.5194/amt-10-1061-2017, https://doi.org/10.5194/amt-10-1061-2017, 2017
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Amines are important drivers in particle formation and growth, which has implications for Earth’s climate. We developed a novel ion chromatographic method for separating and quantifying the 11 most abundant atmospheric alkyl amines, including three sets of structural isomers and two diamines. The detection limits are in the picogram per injection range. We quantified these analytes in two Canadian biomass burning aerosol samples with ammonium ratios of 1 : 2 up to 1000 : 1.
Christoph A. Lehmeier, Ford Ballantyne IV, Kyungjin Min, and Sharon A. Billings
Biogeosciences, 13, 3319–3329, https://doi.org/10.5194/bg-13-3319-2016, https://doi.org/10.5194/bg-13-3319-2016, 2016
S. A. Billings, L. K. Tiemann, F. Ballantyne IV, C. A. Lehmeier, and K. Min
SOIL, 1, 313–330, https://doi.org/10.5194/soil-1-313-2015, https://doi.org/10.5194/soil-1-313-2015, 2015
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We highlight observations relevant to soil organic matter (SOM) decay and retention but often emanating from disparate fields. First, we describe relevant natural and artificial aquatic environments. Second, we describe how intrinsic patterns of decay kinetics for purified soil substrates are useful for defining baseline rates. Third, we describe theoretical advances important for the discipline. Last, we describe how these advances can be used to unravel the mysteries of deep SOM persistence.
Related subject area
Soils and global change
Thermodynamic and hydrological drivers of the soil and bedrock thermal regimes in central Spain
The effect of different biopreparations on soil physical properties and CO2 emissions when growing winter wheat and oilseed rape
Earthworm-invaded boreal forest soils harbour distinct microbial communities
Back to the future? Conservative grassland management can preserve soil health in the changing landscapes of Uruguay
Effects of a warmer climate and forest composition on soil carbon cycling, soil organic matter stability and stocks in a humid boreal region
Effects of mild alternate wetting and drying irrigation and rice straw application on N2O emissions in rice cultivation
Whole-soil warming decreases abundance and modifies the community structure of microorganisms in the subsoil but not in surface soil
Acute glyphosate exposure does not condition the response of microbial communities to a dry–rewetting disturbance in a soil with a long history of glyphosate-based herbicides
Depletion of soil carbon and aggregation after strong warming of a subarctic Andosol under forest and grassland cover
Effect of deforestation and subsequent land use management on soil carbon stocks in the South American Chaco
The effects of worms, clay and biochar on CO2 emissions during production and soil application of co-composts
Climate and soil factors influencing seedling recruitment of plant species used for dryland restoration
A call for international soil experiment networks for studying, predicting, and managing global change impacts
Global distribution of soil organic carbon – Part 2: Certainty of changes related to land use and climate
The economics of soil C sequestration and agricultural emissions abatement
Félix García-Pereira, Jesús Fidel González-Rouco, Thomas Schmid, Camilo Melo-Aguilar, Cristina Vegas-Cañas, Norman Julius Steinert, Pedro José Roldán-Gómez, Francisco José Cuesta-Valero, Almudena García-García, Hugo Beltrami, and Philipp de Vrese
SOIL, 10, 1–21, https://doi.org/10.5194/soil-10-1-2024, https://doi.org/10.5194/soil-10-1-2024, 2024
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This work addresses air–ground temperature coupling and propagation into the subsurface in a mountainous area in central Spain using surface and subsurface data from six meteorological stations. Heat transfer of temperature changes at the ground surface occurs mainly by conduction controlled by thermal diffusivity of the subsurface, which varies with depth and time. A new methodology shows that near-surface diffusivity and soil moisture content changes with time are closely related.
Sidona Buragienė, Egidijus Šarauskis, Aida Adamavičienė, Kęstutis Romaneckas, Kristina Lekavičienė, Daiva Rimkuvienė, and Vilma Naujokienė
SOIL, 9, 593–608, https://doi.org/10.5194/soil-9-593-2023, https://doi.org/10.5194/soil-9-593-2023, 2023
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The aim of this study was to investigate the effects of different biopreparations on soil porosity, temperature, and CO2 emission from the soil in northeast Europe (Lithuania) when growing food-type crops. The application of the biopreparations showed a cumulative effect on the soil properties. In the third year of the study, the total porosity of the soil was higher in all scenarios compared to the control, ranging between 51% and 74%.
Justine Lejoly, Sylvie Quideau, Jérôme Laganière, Justine Karst, Christine Martineau, Mathew Swallow, Charlotte Norris, and Abdul Samad
SOIL, 9, 461–478, https://doi.org/10.5194/soil-9-461-2023, https://doi.org/10.5194/soil-9-461-2023, 2023
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Earthworm invasion in North American forests can alter soil functioning. We investigated how the presence of invasive earthworms affected microbial communities, key drivers of soil biogeochemistry, across the major soil types of the Canadian boreal forest, which is a region largely understudied. Although total microbial biomass did not change, community composition shifted in earthworm-invaded mineral soils, where we also found higher fungal biomass and greater microbial species diversity.
Ina Säumel, Leonardo R. Ramírez, Sarah Tietjen, Marcos Barra, and Erick Zagal
SOIL, 9, 425–442, https://doi.org/10.5194/soil-9-425-2023, https://doi.org/10.5194/soil-9-425-2023, 2023
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We analyzed intensification of Uruguayan grasslands in a country-wide survey on fertility proxies, pH and trace metals in topsoils. We observed a loss of nutrients, trace metals and organic matter in grasslands, croplands and timber plantations and accumulation in riverine forests. This raises questions about the carrying capacity of Uruguayan soils with regard to currently implemented intensification strategies and supports more conservative forms of extensive grassland management.
David Paré, Jérôme Laganière, Guy R. Larocque, and Robert Boutin
SOIL, 8, 673–686, https://doi.org/10.5194/soil-8-673-2022, https://doi.org/10.5194/soil-8-673-2022, 2022
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Major soil carbon pools and fluxes were assessed along a climatic gradient expanding 4 °C in mean annual temperature for two important boreal conifer forest stand types. Species and a warmer climate affected soil organic matter (SOM) cycling but not stocks. Contrarily to common hypotheses, SOM lability was not reduced by warmer climatic conditions and perhaps increased. Results apply to cold and wet conditions and a stable vegetation composition along the climate gradient.
Kaikuo Wu, Wentao Li, Zhanbo Wei, Zhi Dong, Yue Meng, Na Lv, and Lili Zhang
SOIL, 8, 645–654, https://doi.org/10.5194/soil-8-645-2022, https://doi.org/10.5194/soil-8-645-2022, 2022
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We explored the effects of mild alternate wetting and drying (AWD) irrigation combined with rice straw return on N2O emissions and rice yield through rice pot experiments. Mild AWD irrigation significantly increased both N2O and yield-scaled N2O emissions. The addition of rice straw under mild AWD irrigation could promote N2O emissions. Mild AWD irrigation could reduce soil-nitrogen uptake by rice when urea was applied. Mild AWD irrigation reduced rice aboveground biomass but not rice yield.
Cyrill U. Zosso, Nicholas O. E. Ofiti, Jennifer L. Soong, Emily F. Solly, Margaret S. Torn, Arnaud Huguet, Guido L. B. Wiesenberg, and Michael W. I. Schmidt
SOIL, 7, 477–494, https://doi.org/10.5194/soil-7-477-2021, https://doi.org/10.5194/soil-7-477-2021, 2021
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How subsoil microorganisms respond to warming is largely unknown, despite their crucial role in the soil organic carbon cycle. We observed that the subsoil microbial community composition was more responsive to warming compared to the topsoil community composition. Decreased microbial abundance in subsoils, as observed in this study, might reduce the magnitude of the respiration response over time, and a shift in the microbial community will likely affect the cycling of soil organic carbon.
Marco Allegrini, Elena Gomez, and María Celina Zabaloy
SOIL, 6, 291–297, https://doi.org/10.5194/soil-6-291-2020, https://doi.org/10.5194/soil-6-291-2020, 2020
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Research was conducted to assess the response of microbial communities in a soil with a long history of glyphosate-based herbicides to a secondary imposed perturbation (dry–rewetting event). Both perturbations could increase their frequency under current agricultural practices and climate change. The results of this study demonstrate that acute exposure to a glyphosate-based herbicide does not have a conditioning effect on the response of microbial communities to the dry–rewetting event.
Christopher Poeplau, Páll Sigurðsson, and Bjarni D. Sigurdsson
SOIL, 6, 115–129, https://doi.org/10.5194/soil-6-115-2020, https://doi.org/10.5194/soil-6-115-2020, 2020
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Global warming leads to increased mineralisation of soil organic matter, inducing a positive climate–carbon cycle feedback loop. Loss of organic matter can be associated with loss of soil structure. Here we use a strong geothermal gradient to investigate soil warming effects on soil organic matter and structural parameters in subarctic forest and grassland soils. Strong depletion of organic matter caused a collapse of aggregates, highlighting the potential impact of warming on soil function.
Natalia Andrea Osinaga, Carina Rosa Álvarez, and Miguel Angel Taboada
SOIL, 4, 251–257, https://doi.org/10.5194/soil-4-251-2018, https://doi.org/10.5194/soil-4-251-2018, 2018
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The sub-humid Argentine Chaco, originally covered by forest, has been subjected to clearing since the end of the 1970s and replacement of the forest by no-till farming. The organic carbon stock content up to 1 m depth varied as follows: forest > pasture > continuous cropping, with no impact of the number of years under cropping. The incorporation of pastures of warm-season grasses was able to mitigate the decrease of C stocks caused by cropping and so could be considered sustainable management.
Justine Barthod, Cornélia Rumpel, Remigio Paradelo, and Marie-France Dignac
SOIL, 2, 673–683, https://doi.org/10.5194/soil-2-673-2016, https://doi.org/10.5194/soil-2-673-2016, 2016
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In this study we evaluated CO2 emissions during composting of green wastes with clay and/or biochar in the presence and absence of worms, as well as the effect of those amendments on carbon mineralization after application to soil. Our results indicated that the addition of clay or clay–biochar mixture reduced carbon mineralization during co-composting without worms by up to 44 %. In the presence of worms, CO2 emissions during composting increased for all treatments except for the low clay dose.
Miriam Muñoz-Rojas, Todd E. Erickson, Dylan C. Martini, Kingsley W. Dixon, and David J. Merritt
SOIL, 2, 287–298, https://doi.org/10.5194/soil-2-287-2016, https://doi.org/10.5194/soil-2-287-2016, 2016
M. S. Torn, A. Chabbi, P. Crill, P. J. Hanson, I. A. Janssens, Y. Luo, C. H. Pries, C. Rumpel, M. W. I. Schmidt, J. Six, M. Schrumpf, and B. Zhu
SOIL, 1, 575–582, https://doi.org/10.5194/soil-1-575-2015, https://doi.org/10.5194/soil-1-575-2015, 2015
M. Köchy, A. Don, M. K. van der Molen, and A. Freibauer
SOIL, 1, 367–380, https://doi.org/10.5194/soil-1-367-2015, https://doi.org/10.5194/soil-1-367-2015, 2015
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Using ranges for variables in a model of organic C stocks of the top 1m of soil on a global 0.5° grid, we assessed the (un)certainty of changes in stocks over the next 75 years. Changes are more certain where land-use change strongly affects carbon inputs and where higher temperatures and adequate moisture favour decomposition, e.g. tropical mountain forests. Global stocks will increase by 1% with a certainty of 75% if inputs to the soil increase due to CO₂ fertilization of the vegetation.
P. Alexander, K. Paustian, P. Smith, and D. Moran
SOIL, 1, 331–339, https://doi.org/10.5194/soil-1-331-2015, https://doi.org/10.5194/soil-1-331-2015, 2015
Cited articles
Alster, C. J., German, D. P., Lu, Y., and Allison, S. D.: Microbial enzymatic responses
to drought and to nitrogen addition in a southern California grassland, Soil Biol. Biochem., 64,
68–79, https://doi.org/10.1016/j.soilbio.2013.03.034, 2013.
Baggs, E. M.: Soil microbial sources of nitrous oxide: Recent advances in knowledge,
emerging challenges and future direction, Curr. Opin. Environ. Sustain., 3, 321–327,
https://doi.org/10.1016/j.cosust.2011.08.011, 2011.
Bai, E., Li, S., Xu, W., Li, W., Dai, W., and Jiang, P.: A meta-analysis of experimental
warming effects on terrestrial nitrogen pools and dynamics, New Phytol., 199, 431–440,
https://doi.org/10.1111/nph.12252, 2013.
Bengtsson, G. and Bergwall, C.: Fate of 15N labelled nitrate and ammonium in a
fertilized forest soil, Soil Biol. Biochem., 32, 545–557, https://doi.org/10.1016/S0038-0717(99)00183-2,
2000.
Benoit, M., Garnier, J., and Billen, G.: Temperature dependence of nitrous oxide
production of a luvisolic soil in batch experiments, Process Biochem., 50, 79–85,
https://doi.org/10.1016/j.procbio.2014.10.013, 2015.
Billings, S. A.: Nitrous oxide in flux, Nature, 456, 888–889, 2008.
Billings, S. A. and Ballantyne, F.: How interactions between microbial resource demands,
soil organic matter stoichiometry, and substrate reactivity determine the direction and magnitude
of soil respiratory responses to warming, Glob. Change Biol., 19, 90–102,
https://doi.org/10.1111/gcb.12029, 2013.
Billings, S. A. and Tiemann, L. K.: Warming-induced enhancement of soil N2O
efflux linked to distinct response times of genes driving N2O production and
consumption, Biogeochemistry, 119, 371–386, https://doi.org/10.1007/s10533-014-9973-2, 2014.
Blume, E., Bischoff, M., Reichert, J. M., Moorman, T., Konopka, A., and Turco, R. F.:
Surface and subsurface microbial biomass, community structure and metabolic activity as a function
of soil depth and season, Appl. Soil Ecol., 20, 171–181, https://doi.org/10.1016/S0929-1393(02)00025-2,
2002.
Bradford, M. A.: Thermal adaptation of decomposer communities in warming soils,
Front. Microbiol., 4, 1–16, https://doi.org/10.3389/fmicb.2013.00333, 2013.
Braker, G. and Tiedje, J. M.: Nitric Oxide Reductase (norB) Genes from Pure Cultures
and Environmental Samples Nitric Oxide Reductase (norB) Genes from Pure Cultures and Environmental
Samples, Appl. Environ. Microb., 69, 3476–3483, https://doi.org/10.1128/AEM.69.6.3476-3483.2003, 2003.
Buckeridge, K. M.: NLBELT_N2O (Version v.1.0), Zenodo, https://doi.org/10.5281/zenodo.3934598, 2020.
Buckeridge, K. M., Banerjee, S., Siciliano, S. D., and Grogan, P.: The seasonal pattern
of soil microbial community structure in mesic low arctic tundra, Soil Biol. Biochem., 65,
338–347, https://doi.org/10.1016/j.soilbio.2013.06.012, 2013.
Butler, S. M., Melillo, J. M., Johnson, J. E., Mohan, J., Steudler, P. A., Lux, H.,
Burrows, E., Smith, R. M., Vario, C. L., Scott, L., Hill, T. D., Aponte, N., and Bowles, F.: Soil
warming alters nitrogen cycling in a New England forest: Implications for ecosystem function and
structure, Oecologia, 168, 819–828, https://doi.org/10.1007/s00442-011-2133-7, 2012.
Butterbach-Bahl, K. and Dannenmann, M.: Denitrification and associated soil
N2O emissions due to agricultural activities in a changing climate, Curr.
Opin. Environ. Sustain., 3, 389–395, https://doi.org/10.1016/j.cosust.2011.08.004, 2011.
Butterbach-Bahl, K., Baggs, E. M., Dannenmann, M., Kiese, R., and
Zechmeister-Boltenstern, S.: Nitrous oxide emissions from soils: how well do we understand the
processes and their controls?, Philos. Trans. R. Soc. B-Biol. Sci., 368, 20130122,
https://doi.org/10.1098/rstb.2013.0122, 2013.
Cavicchioli, R., Bakken, L. R., Baylis, M., Foreman, C. M., Karl, D. M., Koskella, B.,
Welch, D. B. M., Martiny, J. B. H., Moran, M. A., Rich, V. I., Singh, B. K., Stein, L. Y.,
Stewart, F. J., Sullivan, M. B., Webb, E. A., and Webster, N. S.: Scientists' warning to humanity:
microorganisms and climate change, Nat. Rev. Microbiol., 17, 569–586,
https://doi.org/10.1038/s41579-019-0222-5, 2019.
Chapuis-Lardy, L., Wrage, N., Metay, A., Chotte, J. L., and Bernoux, M.: Soils, a sink
for N2O? A review, Glob. Change Biol., 13, 1–17,
https://doi.org/10.1111/j.1365-2486.2006.01280.x, 2007.
Ciais, P., Sabine, C., Bala, G., Bopp, L., Brovkin, V., Canadell, J., Chhabra, A.,
DeFries, R., Galloway, J., Heimann, M., Jones, C., Le Quéré, C., Myneni, R. B., Piao, S., and Thornton, P.: Carbon and Other Biogeochemical Cycles Supplementary Material, in: Climate Change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change, edited by: Stocker, T. F., Qin, D., Plattner, G.-K., Tignor, M., Allen, S. K., Boschung, J., Nauels, A., Xia, Y., Bex, V., and Midgley, P. M., Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA, 2013.
Cui, P., Fan, F., Yin, C., Song, A., Huang, P., Tang, Y., Zhu, P., Peng, C., Li, T.,
Wakelin, S. A., and Liang, Y.: Long-term organic and inorganic fertilization alters temperature
sensitivity of potential N2O emissions and associated microbes, Soil Biol. Biochem., 93,
131–141, https://doi.org/10.1016/j.soilbio.2015.11.005, 2016.
Delgado-Baquerizo, M., Grinyer, J., Reich, P. B., and Singh, B. K.: Relative importance
of soil properties and microbial community for soil functionality: insights from a microbial swap
experiment, Funct. Ecol., 30, 1862–1873, https://doi.org/10.1111/1365-2435.12674, 2016.
Dijkstra, F. A., Prior, S. A., Runion, G. B., Torbert, H. A., Tian, H., Lu, C., and
Venterea, R. T.: Effects of elevated carbon dioxide and increased temperature on methane and
nitrous oxide fluxes: Evidence from field experiments, Front. Ecol. Environ., 10, 520–527,
https://doi.org/10.1890/120059, 2012.
Enanga, E. M., Creed, I. F., Fairweather, T., and Casson, N. J.: Snow-covered soils
produce N2O that is lost from forested catchments, J. Geophys. Res.-Biogeosci., 121,
2356–2368, https://doi.org/10.1002/2016JG003411, 2016.
Environment and Climate Change Canada: Climate Change Normals 1981–2010 Station Data,
available at: http://climate.weather.gc.ca/climate_normals/, last access: 20 August 2020.
Fierer, N., Schimel, J. P., and Holden, P. A.: Variations in microbial community
composition through two soil depth profiles, Soil Biol. Biochem., 35, 167–176, 2003.
Firestone, M. K. and Davidson, E. A.: Microbiologial Basis of NO and
N2O production and consumption in soil, in: Exchange of Trace Gases between Terrestrial
Ecosystems and the Atmosphere, edited by: Andreae, M. O. and Schimel, D. S., 7–21, Wiley and
Sons Ltd., Bernhard, Dahlem Konferenzen, 1989.
Goldberg, S. D. and Gebauer, G.: Drought turns a Central European Norway spruce forest
soil from an N2O source to a transient N2O sink, Glob. Change Biol., 15,
850–860, https://doi.org/10.1111/j.1365-2486.2008.01752.x, 2009.
Henry, S., Bru, D., Stres, B., Hallet, S., and Philippot, L.: Quantitative detection of
the nosZ gene, encoding nitrous oxide reductase, and comparison of the abundances of 16S rRNA,
narG, nirK, and nosZ genes in soils, Appl. Environ. Microb., 72, 5181–5189,
https://doi.org/10.1128/AEM.00231-06, 2006.
Jones, C. M., Graf, D. R. H., Bru, D., Philippot, L., and Hallin, S.: The unaccounted
yet abundant nitrous oxide-reducing microbial community: A potential nitrous oxide sink, ISME J.,
7, 417–426, https://doi.org/10.1038/ismej.2012.125, 2013.
Jones, C. M., Spor, A., Brennan, F. P., Breuil, M.-C., Bru, D., Lemanceau, P.,
Griffiths, B., Hallin, S., and Philippot, L.: Recently identified microbial guild mediates soil
N2O sink capacity, Nat. Clim. Change, 4, 801–805, https://doi.org/10.1038/nclimate2301, 2014.
Keil, D., Niklaus, P. A., von Riedmatten, L. R., Boeddinghaus, R. S., Dormann, C. F.,
Scherer-Lorenzen, M., Kandeler, E., and Marhan, S.: Effects of warming and drought on potential
N2O emissions and denitrifying bacteria abundance in grasslands with different land-use,
FEMS Microbiol. Ecol., 91, 1–9, https://doi.org/10.1093/femsec/fiv066, 2015.
Kurganova, I. N. and Lopes de Gerenyu, V. O.: Effect of the temperature and moisture on
the N2O emission from some arable soils, Eurasian Soil Sci., 43, 919–928,
https://doi.org/10.1134/S1064229310080090, 2010.
Luckmann, M., Mania, D., Kern, M., Bakken, L. R., Frostegård, A., and Simon, J.:
Production and consumption of nitrous oxide in nitrate-ammonifying Wolinella succinogenes cells,
Microbiology, 160, 1749–1759, https://doi.org/10.1099/mic.0.079293-0, 2014.
McClain, M. E., Boyer, E. W., Dent, C. L., Gergel, S. E., Grimm, N. B., Groffman,
P. M., Hart, S. C., Harvey, J. W., Johnston, C. A., Mayorga, E., McDowell, W. H., and Pinay, G.:
Biogeochemical hot spots and hot moments at the interface of terrestrial and aquatic ecosystems,
Ecosystems, 6, 301–312, https://doi.org/10.1007/s10021-003-0161-9, 2003.
McDaniel, M. D., Kaye, J. P., and Kaye, M. W.: Increased temperature and precipitation
had limited effects on soil extracellular enzyme activities in a post-harvest forest, Soil
Biol. Biochem., 56, 90–98, https://doi.org/10.1016/j.soilbio.2012.02.026, 2013.
Min, K., Buckeridge, K., Ziegler, S. E., Edwards, K. A., Bagchi, S., and Billings,
S. A.: Temperature sensitivity of biomass-specific microbial exo-enzyme activities and CO2
efflux is resistant to change across short- and long-term timescales, Glob. Change Biol., 25,
1793–1807, https://doi.org/10.1111/gcb.14605, 2019.
Morse, J. L., Durán, J., and Groffman, P. M.: Soil Denitrification Fluxes in a
Northern Hardwood Forest: The Importance of Snowmelt and Implications for Ecosystem N
Budgets, Ecosystems, 18, 520–532, https://doi.org/10.1007/s10021-015-9844-2, 2015.
Mosier, A., Kroeze, C., Nevison, C., Oenema, O., and Seitzinger, S.: Closing the global
N2O budget?: nitrous oxide emissions through the agricultural nitrogen cycle inventory
methodology, Nutr. Cycl. Agroecosys., 52, 225–248, https://doi.org/10.1023/A:1009740530221, 1998.
Oh, N.-H., Kim, H.-S., and Richter, D. D.: What Regulates Soil CO2
Concentrations? A Modeling Approach to CO2 Diffusion in Deep Soil Profiles,
Environ. Eng. Sci., 22, 38–45, https://doi.org/10.1089/ees.2005.22.38, 2005.
Pell, M., Stenberg, B., Stenstrom, J., and Torstensson, L.: Potential denitrification
activity assay in soil – With or without chloramphenicol?, Soil Biol. Biochem., 28, 393–398,
https://doi.org/10.1016/0038-0717(95)00149-2, 1996.
Petersen, D. G., Blazewicz, S. J., Firestone, M., Herman, D. J., Turetsky, M., and
Waldrop, M.: Abundance of microbial genes associated with nitrogen cycling as indices of
biogeochemical process rates across a vegetation gradient in Alaska, Environ. Microbiol., 14,
993–1008, https://doi.org/10.1111/j.1462-2920.2011.02679.x, 2012.
Philben, M., Ziegler, S. E., Edwards, K. A., Kahler, R., and Benner, R.: Soil organic
nitrogen cycling increases with temperature and precipitation along a boreal forest latitudinal
transect, Biogeochemistry, 127, 397–410, https://doi.org/10.1007/s10533-016-0187-7, 2016.
Podrebarac, F. A., Laganière, J., Billings, S. A., Edwards, K. A., and Ziegler,
S. E.: Soils isolated during incubation underestimate temperature sensitivity of respiration and
its response to climate history, Soil Biol. Biochem., 93, 60–68,
https://doi.org/10.1016/j.soilbio.2015.10.012, 2016.
Portmann, R. W., Daniel, J. S., and Ravishankara, A. R.: Stratospheric ozone depletion
due to nitrous oxide: influences of other gases, Philos. Trans. R. Soc. B-Biol. Sci., 367,
1256–1264, https://doi.org/10.1098/rstb.2011.0377, 2012.
R Core Team: R: A language and environment for statistical computing, R Foundation for Statistical Computing, Vienna, Austria, 2014.
Richter, D., Richter, D., and Billings, S. A.: Tansley review “One physical system”:
Tansley's ecosystem as Earth's critical zone, New Phytol., 206, 900–912, 2015.
Rösch, C., Mergel, A., Bothe, H., and Ro, C.: Biodiversity of Denitrifying and
Dinitrogen-Fixing Bacteria in an Acid Forest Soil, Appl. Environ. Microb., 68, 3818–3829,
https://doi.org/10.1128/AEM.68.8.3818-3829.2002, 2002.
Schmidt, C. S., Richardson, D. J., and Baggs, E. M.: Constraining the conditions
conducive to dissimilatory nitrate reduction to ammonium in temperate arable soils, Soil
Biol. Biochem., 43, 1607–1611, https://doi.org/10.1016/j.soilbio.2011.02.015, 2011.
Soil Classification Working Group: The Canadian System of Soil Classification, 3rd
Edn., Agric. and Agri-Food Can. Publ. 1646 (Revised), Ottawa, Canada, 1998.
Spott, O., Russow, R., and Stange, C. F.: Formation of hybrid N2O and hybrid
N2 due to codenitrification: First review of a barely considered process of microbially
mediated N-nitrosation, Soil Biol. Biochem., 43, 1995–2011, https://doi.org/10.1016/j.soilbio.2011.06.014,
2011.
Szukics, U., Abell, G. C. J., Hödl, V., Mitter, B., Sessitsch, A., Hackl, E., and
Zechmeister-Boltenstern, S.: Nitrifiers and denitrifiers respond rapidly to changed moisture and
increasing temperature in a pristine forest soil, FEMS Microbiol. Ecol., 72, 395–406,
https://doi.org/10.1111/j.1574-6941.2010.00853.x, 2010.
Throbäck, I. N., Enwall, K., Jarvis, Å., and Hallin, S.: Reassessing PCR
primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with
DGGE, FEMS Microbiol. Ecol., 49, 401–417, https://doi.org/10.1016/j.femsec.2004.04.011, 2004.
Tischer, A., Blagodatskaya, E., and Hamer, U.: Microbial community structure and
resource availability drive the catalytic efficiency of soil enzymes under land-use change
conditions, Soil Biol. Biochem., 89, 226–237, https://doi.org/10.1016/j.soilbio.2015.07.011, 2015.
Uchida, Y. and Clough, T. J.: Nitrous oxide emissions from pastures during wet and cold
seasons, Grassl. Sci., 61, 61–74, https://doi.org/10.1111/grs.12093, 2015.
Venables, W. N. and Ripley, B. D.: Modern Applied Statistics With S, Technometrics, 45,
111–111, https://doi.org/10.1198/tech.2003.s33, 2003.
Wallenstein, M. D. and Vilgalys, R. J.: Quantitative analyses of nitrogen cycling genes
in soils, Pedobiologia (Jena), 49, 665–672, https://doi.org/10.1016/j.pedobi.2005.05.005, 2005.
Wallenstein, M. D., Myrold, D. D., Firestone, M., and Voytek, M.: Environmental
controls on denitrifying communities and denitrification rates?: Insights from molecular methods,
Ecol. Appl., 16, 2143–2152, 2006.
Wallenstein, M. D., Haddix, M. L., Ayres, E., Steltzer, H., Magrini-Bair, K. A., and
Paul, E. A.: Litter chemistry changes more rapidly when decomposed at home but converges during
decomposition-transformation, Soil Biol. Biochem., 57, 311–319,
https://doi.org/10.1016/j.soilbio.2012.09.027, 2013.
Wang, J., Song, C., Zhang, J., Wang, L., Zhu, X., and Shi, F.: Temperature sensitivity
of soil carbon mineralization and nitrous oxide emission in different ecosystems along a mountain
wetland-forest ecotone in the continuous permafrost of Northeast China, Catena, 121, 110–118,
https://doi.org/10.1016/j.catena.2014.05.007, 2014.
Ziegler, S. E., Benner, R., Billings, S. A., Edwards, K. A., Philben, M., Zhu, X., and
Laganière, J.: Climate warming can accelerate carbon fluxes without changing soil carbon
stocks, Front. Earth Sci., 5, 2, https://doi.org/10.3389/feart.2017.00002, 2017.
Short summary
We do not understand the short- and long-term temperature response of soil denitrifiers, which produce and consume N2O. Boreal forest soils from a long-term climate gradient were incubated in short-term warming experiments. We found stronger N2O consumption at depth, inconsistent microbial gene abundance and function, and consistent higher N2O emissions from warmer-climate soils at warmer temperatures. Consideration of our results in models will contribute to improved climate projections.
We do not understand the short- and long-term temperature response of soil denitrifiers, which...