Dear Drs. McDaniel & Grandy,
Thank you for your careful and thorough responses to the reviewers’ comments. You have clearly addressed the major concerns of all reviewers, and I have no additional recommendations or concerns. I am inviting you to upload the revised manuscript for a final review by myself. I look forward to reading the revised manuscript.

Dear Dr. McDaniel & Grandy,

Thank you for your careful revision of the manuscript “Soil microbial biomass and function are altered by 12 years of crop rotation.” I have consulted with the original reviewers and we find the manuscript is greatly improved and is acceptable for publication in SOIL. I have found a few minor technical errors that should be corrected before final publications, see below. Congratulations and best wishes with your continued work!

Minor technical corrections:
Line 108: citation: (KBS, 2012) Is this supposed to be 2015? Elsewhere in the paper, including the reference section, this seems to be 2015 (for the date accessed)

Line 168: citation: (Lee et al. 2006). I believe this is 2007, it is correct in the bibliography

Line 263-265: It may be helpful to mention the qCO2 in the methods, right now it only appears in the Results section.

Line 407-409: which methods are used to assess microbial functional diversity in these three studies? are the same as the method used in this study?

Line 432: whose preference for C substrates? Microbes?

Line 564-565: I believe this paper is from 1995, the in-text citation is correct

Line 609-610: I believe this paper is from 2014, both the reference and the in-text citation should be updated.

Line 766-767: I believe this paper is from 2010, the in-text citation is correct

Dear Authors,

We are pleased to accept your paper for publication in SOIL after the minor technical corrections required by the Topical Editor.

We look forward to work with you again on future publications.