Articles | Volume 2, issue 4
https://doi.org/10.5194/soil-2-499-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/soil-2-499-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
Original research article
| 
04 Oct 2016
Original research article |
| 04 Oct 2016
Enzymatic biofilm digestion in soil aggregates facilitates the release of particulate organic matter by sonication
Frederick Büks
CORRESPONDING AUTHOR
Chair of Soil Science, Department of Ecology, Technische
Universität Berlin, Berlin, Germany
Martin Kaupenjohann
Chair of Soil Science, Department of Ecology, Technische
Universität Berlin, Berlin, Germany
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Cited
19 citations as recorded by crossref.
- Keystone species determine the “selection mechanism” of multispecies biofilms for bacteria from soil aggregates X. Xiong et al. https://doi.org/10.1016/j.scitotenv.2021.145069
- Characterising soil extracellular polymeric substances (EPS) by application of spectral-chemometrics and deconstruction of the extraction process M. Zhang et al. https://doi.org/10.1016/j.chemgeo.2022.121271
- Soil microbial EPS resiliency is influenced by carbon source accessibility A. Bhattacharjee et al. https://doi.org/10.1016/j.soilbio.2020.108037
- A chronosequence of soil health under tallgrass prairie reconstruction C. Li et al. https://doi.org/10.1016/j.apsoil.2021.103939
- Micro-Scale Microbial Dynamics at the Soil–Water Interface: Biofilm Architecture, Non-Linear Response, and Emerging Methodological Frontiers A. Majumdar et al. https://doi.org/10.3390/w18060658
- Physical protection of extracellular and intracellular DNA in soil aggregates against simulated natural oxidative processes S. Pathan et al. https://doi.org/10.1016/j.apsoil.2021.104002
- Unifying soil organic matter formation and persistence frameworks: the MEMS model A. Robertson et al. https://doi.org/10.5194/bg-16-1225-2019
- Impact of plastic pollution on agricultural soil: analysing extracellular polymeric substances (EPS) and morphological changes in microplastics with sludge amendments P. Singh et al. https://doi.org/10.1007/s11368-026-04404-8
- Discovering Polyphosphate and Polyhydroxyalkanoate-Accumulating Organisms Across Ecosystems: Phenotype-Targeted Genotyping via FACS-Sequencing Y. Yan et al. https://doi.org/10.1021/acs.est.5c07205
- Soil biofilms: microbial interactions, challenges, and advanced techniques for ex-situ characterization P. Cai et al. https://doi.org/10.1007/s42832-019-0017-7
- Technical note: The recovery rate of free particulate organic matter from soil samples is strongly affected by the method of density fractionation F. Büks https://doi.org/10.5194/bg-20-1529-2023
- Bacterial Extracellular Polymeric Substances Amplify Water Content Variability at the Pore Scale Y. Guo et al. https://doi.org/10.3389/fenvs.2018.00093
- The incubation history of soil samples strongly affects the occlusion of particulate organic matter F. Büks et al. https://doi.org/10.5194/bg-22-4679-2025
- Microbial aggregates and functional materials for mitigating soil nitrogen loss: a review N. Li et al. https://doi.org/10.1007/s11157-025-09754-2
- Interface interaction between yeast and β-glucan drives soil aggregate stabilization: molecular mechanisms and bioremediation functions Y. Yan et al. https://doi.org/10.1016/j.jclepro.2026.149308
- Particles under stress: ultrasonication causes size and recovery rate artifacts with soil-derived POM but not with microplastics F. Büks et al. https://doi.org/10.5194/bg-18-159-2021
- Microbial extracellular polymeric substances in the environment, technology and medicine H. Flemming et al. https://doi.org/10.1038/s41579-024-01098-y
- Extraction of extracellular polymeric substances (EPS) from red soils (Ultisols) S. Wang et al. https://doi.org/10.1016/j.soilbio.2019.05.014
- An invisible workforce in soil: The neglected role of soil biofilms in conjugative transfer of antibiotic resistance genes S. Wu et al. https://doi.org/10.1080/10643389.2021.1892015
19 citations as recorded by crossref.
- Keystone species determine the “selection mechanism” of multispecies biofilms for bacteria from soil aggregates X. Xiong et al. https://doi.org/10.1016/j.scitotenv.2021.145069
- Characterising soil extracellular polymeric substances (EPS) by application of spectral-chemometrics and deconstruction of the extraction process M. Zhang et al. https://doi.org/10.1016/j.chemgeo.2022.121271
- Soil microbial EPS resiliency is influenced by carbon source accessibility A. Bhattacharjee et al. https://doi.org/10.1016/j.soilbio.2020.108037
- A chronosequence of soil health under tallgrass prairie reconstruction C. Li et al. https://doi.org/10.1016/j.apsoil.2021.103939
- Micro-Scale Microbial Dynamics at the Soil–Water Interface: Biofilm Architecture, Non-Linear Response, and Emerging Methodological Frontiers A. Majumdar et al. https://doi.org/10.3390/w18060658
- Physical protection of extracellular and intracellular DNA in soil aggregates against simulated natural oxidative processes S. Pathan et al. https://doi.org/10.1016/j.apsoil.2021.104002
- Unifying soil organic matter formation and persistence frameworks: the MEMS model A. Robertson et al. https://doi.org/10.5194/bg-16-1225-2019
- Impact of plastic pollution on agricultural soil: analysing extracellular polymeric substances (EPS) and morphological changes in microplastics with sludge amendments P. Singh et al. https://doi.org/10.1007/s11368-026-04404-8
- Discovering Polyphosphate and Polyhydroxyalkanoate-Accumulating Organisms Across Ecosystems: Phenotype-Targeted Genotyping via FACS-Sequencing Y. Yan et al. https://doi.org/10.1021/acs.est.5c07205
- Soil biofilms: microbial interactions, challenges, and advanced techniques for ex-situ characterization P. Cai et al. https://doi.org/10.1007/s42832-019-0017-7
- Technical note: The recovery rate of free particulate organic matter from soil samples is strongly affected by the method of density fractionation F. Büks https://doi.org/10.5194/bg-20-1529-2023
- Bacterial Extracellular Polymeric Substances Amplify Water Content Variability at the Pore Scale Y. Guo et al. https://doi.org/10.3389/fenvs.2018.00093
- The incubation history of soil samples strongly affects the occlusion of particulate organic matter F. Büks et al. https://doi.org/10.5194/bg-22-4679-2025
- Microbial aggregates and functional materials for mitigating soil nitrogen loss: a review N. Li et al. https://doi.org/10.1007/s11157-025-09754-2
- Interface interaction between yeast and β-glucan drives soil aggregate stabilization: molecular mechanisms and bioremediation functions Y. Yan et al. https://doi.org/10.1016/j.jclepro.2026.149308
- Particles under stress: ultrasonication causes size and recovery rate artifacts with soil-derived POM but not with microplastics F. Büks et al. https://doi.org/10.5194/bg-18-159-2021
- Microbial extracellular polymeric substances in the environment, technology and medicine H. Flemming et al. https://doi.org/10.1038/s41579-024-01098-y
- Extraction of extracellular polymeric substances (EPS) from red soils (Ultisols) S. Wang et al. https://doi.org/10.1016/j.soilbio.2019.05.014
- An invisible workforce in soil: The neglected role of soil biofilms in conjugative transfer of antibiotic resistance genes S. Wu et al. https://doi.org/10.1080/10643389.2021.1892015
Saved (final revised paper)
Latest update: 19 Sep 2026
Short summary
Soil aggregate stability and POM occlusion are integral markers for soil quality. Besides physico-chemical interactions, biofilms are considered to aggregate primary particles, but experimental proof is still missing. In our experiment, soil aggregate samples were treated with biofilm degrading enzymes and showed a reduced POM occlusion and an increased bacteria DNA release compared with untreated samples. Thus, biofilms are assumed to be an important factor of POM occlusion in soil aggregates.
Soil aggregate stability and POM occlusion are integral markers for soil quality. Besides...
