Articles | Volume 12, issue 2
https://doi.org/10.5194/soil-12-947-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/soil-12-947-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Unraveling the plant growth promotion potential of Pseudomonas species isolated from the rhizosphere of Lotus creticus grown in the Mediterranean coastal regions of Morocco
Imane Achkouk
CORRESPONDING AUTHOR
Research Team of Biotechnology and Biomolecular Engineering, FST of Tangier, 90000, Abdelmalek Essaadi University, Tétouan, Morocco
Anass El Yemlahi
Research Team of Biotechnology and Biomolecular Engineering, FST of Tangier, 90000, Abdelmalek Essaadi University, Tétouan, Morocco
Ouiam El Galiou
Research Team of Biotechnology and Biomolecular Engineering, FST of Tangier, 90000, Abdelmalek Essaadi University, Tétouan, Morocco
Amin Laglaoui
Research Team of Biotechnology and Biomolecular Engineering, FST of Tangier, 90000, Abdelmalek Essaadi University, Tétouan, Morocco
Mounir Hassani Zerrouk
Research Team of Biotechnology and Biomolecular Engineering, FST of Tangier, 90000, Abdelmalek Essaadi University, Tétouan, Morocco
Mohammed Bakkali
Research Team of Biotechnology and Biomolecular Engineering, FST of Tangier, 90000, Abdelmalek Essaadi University, Tétouan, Morocco
Abdelhay Arakrak
Research Team of Biotechnology and Biomolecular Engineering, FST of Tangier, 90000, Abdelmalek Essaadi University, Tétouan, Morocco
Cited articles
Abd-Alla, M. H., Al-Amri, S. M., and El-Enany, A.-W. E.: Enhancing rhizobium-legume symbiosis and reducing nitrogen fertilizer use are potential options for mitigating climate change, Agriculture, 13, 2092, https://doi.org/10.3390/agriculture13112092, 2023.
Adamczyk, B.: Root-derived proteases as a plant tool to access soil organic nitrogen; current stage of knowledge and controversies, Plants, 10, 731, https://doi.org/10.3390/plants10040731, 2021.
Adeleke, B. S., Chaudhary, P., Ayilara, M. S., Ojo, F. M., Erinoso, S. M., Upadhayay, V. K., Adeyemo, A. I., and Akinola, S. A.: Rhizosphere microbiomes mediating abiotic stress mitigation for improved plant nutrition, Ecologies, 5, 375–401, https://doi.org/10.3390/ecologies5030024, 2024.
Ainane, A., Abdoul-Latif, F. M., and Ainane, T.: Culture des plantes légumineuses en Afrique: un système agricole durable, Éditions Universitaires Européennes, ISBN-13 978-620-341-419-6, 2021.
Ames, B. N.: Assay of inorganic phosphate, total phosphate and phosphatases, Methods Enzymol., 8, 115–118, https://doi.org/10.1016/0076-6879(66)08014-5, 1966.
AOAC: Official methods of analysis, 16th Edn., Association of Official Analytical Chemists, Gaithersburg, MD, USA, ISBN 978-0-935584-54-7, 1997.
Araniti, F., Sunseri, F., and Abenavoli, M. R.: Phytotoxic activity and phytochemical characterization of Lotus ornithopodioides L., a spontaneous species of the Mediterranean area, Phytochem. Lett., 8, 179–183, 2014.
Bakker, A. W. and Schippers, B.: Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation, Soil Biol. Biochem., 19, 451–457, https://doi.org/10.1016/0038-0717(87)90037-X, 1987.
Barreira, J. C., Visnevschi-Necrasov, T., Pereira, G., Nunes, E., and Oliveira, M. B. P.: Phytochemical profiling of underexploited Fabaceae species: insights on ontogenic and phylogenetic effects over isoflavone levels, Food Res. Int., 100, 517–523, https://doi.org/10.1016/j.foodres.2016.07.009, 2017.
Bashan, Y., de-Bashan, L. E., Prabhu, S. R., and Hernandez, J.-P.: Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013), Plant Soil, 378, 1–33, https://doi.org/10.1007/s11104-013-1956-x, 2014.
Belechheb, T., Bouhnik, O., Bakkali, M., Hassani Zerrouk, M., Laglaoui, A., Missbah El Idrissi, M., and Arakrak, A.: Ensifer meliloti sv. lancerottense nodulates Lotus creticus in alkaline soils of Northern Morocco, Rhizosphere, 18, 100339, https://doi.org/10.1016/j.rhisph.2021.100339, 2021.
Bower, C. A., Reitemeier, R. F., and Fireman, M.: Exchangeable cation analysis of saline and alkali soils, Soil Sci., 73, 251–262, https://doi.org/10.1097/00010694-195204000-00001, 1952.
Bric, J. M., Bostock, R. M., and Silverstone, S. E.: Rapid in situ assay for indoleacetic acid production by bacteria immobilized on nitrocellulose membrane, Appl. Environ. Microbiol., 57, 535–538, 1991.
Brink, B.: Urease test protocol, American Society for Microbiology, https://asm.org/protocols/urease-test (last access: 16 February 2026), 2010.
Broughton, W. J. and Dilworth, M. J.: Control of leghaemoglobin synthesis in snake beans, Biochem. J., 125, 1075–1080, https://doi.org/10.1042/bj1251075, 1971.
Bruning, B. and Rozema, J.: Symbiotic nitrogen fixation in legumes: perspectives for saline agriculture, Environ. Exp. Bot., 92, 134–143, https://doi.org/10.1016/j.envexpbot.2012.09.001, 2013.
Bumunang, W. E. and Babalola, O. O.: Characterization of rhizobacteria from field grown genetically modified (GM) and non-GM maizes, Braz. Arch. Biol. Technol., 57, https://doi.org/10.1590/S1516-89132014000100001, 2014.
Cappuccino, J. G. and Sherman, N.: Microbiology: a laboratory manual, 3rd Edn., Benjamin/Cummings, New York, USA, ISBN 978-0-8053-1052-8, 1992.
Chen, W. P. and Kuo, T. T.: A simple and rapid method for the preparation of Gram-negative bacterial genomic DNA, Nucleic Acids Res., 21, 2260, https://doi.org/10.1093/nar/21.9.2260, 1993.
Chu, W., Shen, C., Zhou, L., Zhang, Y., Li, X., Wang, H., Liu, J., and Chen, Q.: Rationalizing microbial strategies for coastal soil restoration: functional complementarity and trade-offs in PGPR-AMF interactions, Land Degrad. Dev., 1–14, https://doi.org/10.1002/ldr.70366, 2025.
Collins, H. C., Lyne, P. M., Grange, J. M., and Falkinham, J. O.: Collins and Lyne’s Microbiological Methods, 8th Edn., Arnold, London, ISBN 978-0-340-80896-2, 2004.
Compant, S., Clément, C., and Sessitsch, A.: Plant growth-promoting bacteria in the rhizo- and endosphere of plants: their role, colonization mechanisms involved and prospects for utilization, Soil Biol. Biochem., 42, 669–678, https://doi.org/10.1016/j.soilbio.2009.11.024, 2010.
Crabtree, K. T. and Hinsdill, R. D.: Fundamental Experiments in Microbiology, W. B. Saunders, Philadelphia, USA, ISBN 978-0-7216-2733-5, 1974.
Datta, R.: Enzymatic degradation of cellulose in soil: a review, Heliyon, 10, e24022, https://doi.org/10.1016/j.heliyon.2024.e24022, 2024.
de Andrade, L. A., Santos, C. H. B., Frezarin, E. T., Sales, L. R., and Rigobelo, E. C.: Plant growth-promoting rhizobacteria for sustainable agricultural production, Microorganisms, 11, 1088, https://doi.org/10.3390/microorganisms11041088, 2023.
Ed-Premono, M., Moawad, M. A., and Vleck, L. G.: Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere, Indones. J. Crop Sci., 11, 13–23, 1996.
El Aaraj, C., Bakkali, M., Infantino, A., Arakrak, A., and Laglaoui, A.: Mycotoxigenic fungi in cereal grains and coffee from the north of Morocco, Am. J. Res. Commun., 3, 2325–4076, 2015.
Espinosa-Palomeque, B., Jiménez-Pérez, O., Ramírez-Gottfried, R. I., Preciado-Rangel, P., Buendía-García, A., Sifuentes, G. Z., Sariñana-Navarrete, M. A., and Rivas-García, T.: Biocontrol of phytopathogens using plant growth-promoting rhizobacteria: bibliometric analysis and systematic review, Horticulturae, 11, 271, https://doi.org/10.3390/horticulturae11030271, 2025.
Etesami, H. and Glick, B. R.: Bacterial indole-3-acetic acid: a key regulator for plant growth, plant-microbe interactions, and agricultural adaptive resilience, Microbiol. Res., 281, 127602, https://doi.org/10.1016/j.micres.2024.127602, 2024.
FAO: Physical and chemical methods of soil and water analysis, Soils Bulletin, 10, Food and Agriculture Organization of the United Nations, Rome, Italy, https://asm.org/protocols/urease-test (last access: 10 August 2026), 1970.
Ferreira, C. S. S., Seifollahi-Aghmiuni, S., Destouni, G., Ghajarnia, N., and Kalantari, Z.: Soil degradation in the European Mediterranean region: processes, status and consequences, Sci. Total Environ., 805, 150106, https://doi.org/10.1016/j.scitotenv.2021.150106, 2022.
Gamalero, E., Lingua, G., and Glick, B. R.: Ethylene, ACC, and the plant growth-promoting enzyme ACC deaminase, Biology, 12, 1043, https://doi.org/10.3390/biology12081043, 2023.
Gerke, J.: Improving phosphate acquisition from soil via higher plants while approaching peak phosphorus worldwide: a critical review of current concepts and misconceptions, Plants, 13, 3478, https://doi.org/10.3390/plants13243478, 2024.
Glick, B. R.: Plant growth-promoting bacteria: mechanisms and applications, Scientifica, 2012, 963401, https://doi.org/10.6064/2012/963401, 2012.
Howieson, J. G. and Dilworth, M. J.: Working with rhizobia, ACIAR Monograph No. 173, Australian Centre for International Agricultural Research, Canberra, Australia, ISBN 978-1-925436-18-1, 2016.
Howieson, J. G., Ballard, R. A., Yates, R. J., Nandasena, K. G., O'Hara, G. W., Tiwari, R. P., Sezmis, E., and Aitken, M.: Selecting improved Lotus-nodulating rhizobia to expedite the development of new forage species, Plant Soil, 348, 231–243, https://doi.org/10.1007/s11104-011-0921-9, 2011.
ISO (International Organization for Standardization): Soil quality – determination of total nitrogen – modified Kjeldahl method (ISO 11261), ISO, Geneva, Switzerland, https://www.iso.org/standard/19239.html (last access: 10 August 2026), 1995.
Jacobson, C. B., Pasternak, J. J., and Glick, B. R.: Partial purification and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2, Can. J. Microbiol., 40, 1019–1025, 1994.
Johan, P. D., Ahmed, O. H., Omar, L., and Hasbullah, N. A.: Phosphorus transformation in soils following co-application of charcoal and wood ash, Agronomy, 11, 2010, https://doi.org/10.3390/agronomy11102010, 2021.
Khan, H., Akbar, W. A., Shah, Z., Rahim, H. U., Taj, A., and Alatalo, J. M.: Coupling phosphate-solubilizing bacteria with inorganic phosphorus fertilizer improves mungbean phosphorus acquisition, nitrogen fixation, and yield in alkaline-calcareous soil, Heliyon, 8, e09081, https://doi.org/10.1016/j.heliyon.2022.e09081, 2022.
Kong, J., Dong, Y., Xu, L., Liu, S., and Bai, X.: Effects of exogenous salicylic acid on alleviating chlorosis induced by iron deficiency in peanut seedlings (Arachis hypogaea L.), J. Plant Growth Regul., 33, 715–729, https://doi.org/10.1007/s00344-014-9417-9, 2014.
Kurepa, J. and Smalle, J. A.: Plant hormone regulation of competitive growth: implications for agriculture and inclusive fitness, Appl. Biosci., 5, 24, https://doi.org/10.3390/applbiosci5020024, 2026.
Lugtenberg, B. and Kamilova, F.: Plant-growth-promoting rhizobacteria, Annu. Rev. Microbiol., 63, 541–556, https://doi.org/10.1146/annurev.micro.62.081307.162918, 2009.
Maciá-Vicente, J. G., Jansson, H.-B., Abdullah, S. K., Descals, E., Salinas, J., and Lopez-Llorca, L. V.: Fungal root endophytes from natural vegetation in Mediterranean environments with special reference to Fusarium spp., FEMS Microbiol. Ecol., 64, 90–105, https://doi.org/10.1111/j.1574-6941.2007.00443.x, 2008.
Madhaiyan, M., Poonguzhali, S., Saravanan, V. S., Lee, J. S., Lee, K. C., and Sundaram, S.: Pseudomonas sesami sp. nov., a plant growth-promoting Gammaproteobacterium isolated from the rhizosphere of Sesamum indicum L., Antonie van Leeuwenhoek, 110, 843–852, https://doi.org/10.1007/s10482-017-0859-x, 2017.
Marien, L., Crabit, A., Dewandel, B., Ladouche, B., Fleury, P., Follain, S., Cavero, J., Berteloot, V., and Colin, F.: Salinity spatial patterns in Mediterranean coastal areas: the legacy of historical water infrastructures, Sci. Total Environ., 895, 165730, https://doi.org/10.1016/j.scitotenv.2023.165730, 2023.
Mekureyaw, M. F., Pandey, C., Hennessy, R. C., Nicolaisen, M. H., Liu, F., Nybroe, O., and Roitsch, T.: The cytokinin-producing plant beneficial bacterium Pseudomonas fluorescens G20-18 primes tomato (Solanum lycopersicum) for enhanced drought stress responses, J. Plant Physiol., 270, 153629, https://doi.org/10.1016/j.jplph.2022.153629, 2022.
Miller, G. L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem., 31, 426–428, https://doi.org/10.1021/ac60147a030, 1959.
Morbia, M., Pandey, A., Mahla, P., and Gohil, S.: Isolation of α-amylase producing microorganisms from soil of Kachchh, Gujarat, J. Pure Appl. Microbiol., 18, 1610–1619, https://doi.org/10.22207/JPAM.18.3.10, 2024.
Msimbira, L. A. and Smith, D. L.: The roles of plant growth-promoting microbes in enhancing plant tolerance to acidity and alkalinity stresses, Front. Sustain. Food Syst., 4, 106, https://doi.org/10.3389/fsufs.2020.00106, 2020.
Navarro-Torre, S., Garcia-Caparrós, P., Nogales, A., Abreu, M. M., Santos, E., Cortinhas, A. L., and Caperta, A. D.: Sustainable agricultural management of saline soils in arid and semi-arid Mediterranean regions through halophytes, microbial and soil-based technologies, Environ. Exp. Bot., https://doi.org/10.1016/j.envexpbot.2023.105397, 2023.
Nisa, M. U., Rizvi, Z. F., Hyder, S., Gondal, A. S., Ahmed, I., Riaz, N., Iqra, Montoya Martínez, A. C., de los Santos-Villalobos, S., and Iqbal, M.: Integrated application of plant growth-promoting rhizobacteria and organic amendments enhances growth and nutrient uptake in maize, J. Agric. Food Res., 102742, https://doi.org/10.1016/j.jafr.2026.102742, 2026.
Novitsky, T. J. and Kushner, D. J.: Influence of temperature and salt concentration on the growth of a facultatively halophilic Micrococcus sp., Can. J. Microbiol., 21, 107–110, https://doi.org/10.1139/m75-017, 1975.
Olsen, S. R., Cole, C. V., Watanabe, F. S., and Dean, L. A.: Estimation of available phosphorus in soils by extraction with sodium bicarbonate, USDA Circular 939, U.S. Department of Agriculture, Washington, DC, 19 pp., https://archive.org/details/estimationofavai939olse (last access: 16 February 2026), 1954.
Orozco-Mosqueda, M. del C., Glick, B. R., and Santoyo, G.: ACC deaminase in plant growth-promoting bacteria (PGPB): an efficient mechanism to counter salt stress in crops, Microbiol. Res., 235, 126439, https://doi.org/10.1016/j.micres.2020.126439, 2020.
Paravar, A., Piri, R., Balouchi, H., and Ma, Y.: Microbial seed coating: an attractive tool for sustainable agriculture, Biotechnol. Rep., 37, e00781, https://doi.org/10.1016/j.btre.2023.e00781, 2023.
Pattnaik, S., Mohapatra, B., and Gupta, A.: Plant growth-promoting microbe-mediated uptake of essential nutrients (Fe, P, K) for crop stress management: microbe-soil-plant continuum, Front. Agron., 3, 689972, https://doi.org/10.3389/fagro.2021.689972, 2021.
Pikovskaya, R. I.: Mobilization of phosphorus in soil in connection with the vital activity of some microbial species, Mikrobiologiya, 17, 362–370, 1948.
Rabindran, R. and Vidhyasekaran, P.: Development of a formulation of Pseudomonas fluorescens PfALR2 for management of rice sheath blight, Crop Prot., 15, 715–721, https://doi.org/10.1016/S0261-2194(96)00045-2, 1996.
Renwick, A., Campbell, R., and Coe, S.: Assessment of in vivo screening systems for potential biocontrol agents of Gaeumannomyces graminis, Plant Pathol., 40, 524–532, 1991.
Rima, F. S., Biswas, S., Sarker, P. K., Islam, M. R., and Seraj, Z. I.: Bacteria endemic to the saline coastal belt and their ability to mitigate the effects of salt stress on rice growth and yields, Ann. Microbiol., 68, 525-535, https://doi.org/10.1007/s13213-018-1358-7, 2018.
Roca-Couso, R., Flores-Félix, J. D., and Rivas, R.: Mechanisms of action of microbial biocontrol agents against Botrytis cinerea, J. Fungi, 7, 1045, https://doi.org/10.3390/jof7121045, 2021.
Romano, G., Ricci, G. F., and Leronni, V.: Soil bioengineering techniques for Mediterranean coastal dune restoration using autochthonous vegetation species, J. Coast. Conserv., 26, 71, https://doi.org/10.1007/s11852-022-00912-0, 2022.
Roy, S., Bhowmik, S., Dutta Chowdhury, A., Dhara, B., and Mitra, A. K.: Plant growth-promoting rhizobacteria: an alternative for NPK fertilizers, in: Microbes and Microbial Biotechnology for Green Remediation, edited by: Malik, J. A., Elsevier, 149–167, https://doi.org/10.1016/B978-0-323-90452-0.00007-4, 2022.
Salwan, I., Sharma, M., Sharma, A., and Sharma, V.: Insights into plant beneficial microorganism-triggered induced systemic resistance, Plant Stress, 7, 100140, https://doi.org/10.1016/j.stress.2023.100140, 2023.
Schwyn, B. and Neilands, J. B.: Universal chemical assay for the detection and determination of siderophores, Anal. Biochem., 160, 47-56, https://doi.org/10.1016/0003-2697(87)90612-9, 1987.
Sehrawat, A., Sindhu, S. S., and Glick, B. R.: Hydrogen cyanide production by soil bacteria: biological control of pests and promotion of plant growth in sustainable agriculture, Pedosphere, 32, 15-38, https://doi.org/10.1016/S1002-0160(21)60058-9, 2022.
Sharma, N., Mahawar, L., Mishra, A., and Albrectsen, B. R.: Microbial contributions to plant growth and stress tolerance: mechanisms for sustainable plant production, Plant Stress, 17, 100966, https://doi.org/10.1016/j.stress.2025.100966, 2025.
Singh, P., Chauhan, P. K., Upadhyay, S. K., Singh, R. K., Dwivedi, P., Wang, J., Jain, D., and Jiang, M.: Mechanistic insights and potential use of siderophore producing microbes in rhizosphere for mitigation of stress in plants grown in degraded land, Front. Microbiol., 13, 898979, https://doi.org/10.3389/fmicb.2022.898979, 2022.
Song, Q., Qi, Z., Liang, N., Hou, N., Zhang, C., Pei, C., Zhao, X., and Li, D.: Cross-protection and cross-feeding mediated by signaling molecules enhanced the viability of Pseudomonas fluorescens S01 and Rhodococcus erythropolis S02 under the dual stresses of polycyclic aromatic hydrocarbons and low temperature, J. Hazard. Mater., 494, 138689, https://doi.org/10.1016/j.jhazmat.2025.138689, 2025.
Sun, Q., Chen, C., Yao, Y., Wu, H., Zhang, M., Jin, L., Zhou, H., Meng, T., and Peng, H.: Soil properties of reclaimed coastal saline-alkali farmland in a Chinese province: spatial variability and soil profiles, Agriculture, 16, 638, https://doi.org/10.3390/agriculture16060638, 2026.
Timofeeva, A. M., Galyamova, M. R., and Sedykh, S. E.: Bacterial siderophores: classification, biosynthesis, perspectives of use in agriculture, Plants, 11, 3065, https://doi.org/10.3390/plants11223065, 2022.
Tsitsigiannis, D. I., Dimakopoulou, M., Antoniou, P. P., and Tjamos, S. E.: Biological control strategies of mycotoxigenic fungi and associated mycotoxins in Mediterranean basin crops, Phytopathol. Mediterr., 51, 158–174, https://doi.org/10.14601/Phytopathol_Mediterr-11502, 2012.
USSLS (United States Salinity Laboratory Staff): Diagnosis and improvement of saline and alkali soils, USDA Handbook No. 60, U.S. Department of Agriculture, Washington, DC, USA, https://www.ars.usda.gov/pacific-west-area/riverside-ca/agricultural-water-efficiency-and-salinity-research-unit/docs/publications/handbook-no-60/ (last access: 10 August 2026), 1954.
Vejan, P., Abdullah, R., Khadiran, T., Ismail, S., and Nasrulhaq Boyce, A.: Role of plant growth promoting rhizobacteria in agricultural sustainability: a review, Molecules, 21, 573, https://doi.org/10.3390/molecules21050573, 2016.
Veliz, E. A., Martínez-Hidalgo, P., and Hirsch, A. M.: Chitinase-producing bacteria and their role in biocontrol, AIMS Microbiol., 3, 689–705, https://doi.org/10.3934/microbiol.2017.3.689, 2017.
Vijayaraghavan, R. and Vincent, S. G. P.: A simple method for the detection of protease activity on agar plates using bromocresol green dye, J. Biochem. Technol., 4, 628–630, 2013.
Walkley, A. and Black, I. A.: An examination of the method for determining soil organic matter and a proposed modification of the chromic acid titration method, Soil Sci., 37, 29–38, https://doi.org/10.1097/00010694-193401000-00003, 1934.
Wang, X., Xu, L., Qi, X., Huang, J., Han, M., Wang, C., Li, X., and Jiang, H.: Microbial assembly and stress-tolerance mechanisms in salt-adapted plants along the shore of a salt lake: implications for saline-alkaline soil remediation, Microorganisms, 13, 1942, https://doi.org/10.3390/microorganisms13081942, 2025.
Weisburg, W. G., Barns, S. M., Pelletier, D. A., and Lane, D. J.: 16S ribosomal DNA amplification for phylogenetic study, J. Bacteriol., 173, 697–703, https://doi.org/10.1128/jb.173.2.697-703.1991, 1991.
Witzel, K., Motos, J. R. A., Atay, E., Hirt, H., and Schmülling, T.: Leveraging microorganisms and biostimulants: mitigating salinity stress in crops with agricultural biologicals, Plant Soil, https://doi.org/10.1007/s11104-025-07578-1, 2025.
Yamagata, A., Murata, Y., Namba, K., Terada, T., Fukai, S., and Shirouzu, M.: Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter, Nat. Commun., 13, 7180, https://doi.org/10.1038/s41467-022-34930-1, 2022.
Yang, J., Tang, M., and Zhao, H.: Physiological mechanisms of plant growth-promoting rhizobacteria in enhancing abiotic stress tolerance of vegetable crops: a review, Plants, 15, 686, https://doi.org/10.3390/plants15050686, 2026.
Yang, Z., Yan, H., Liu, H., Yang, L., Mi, G., and Wang, P.: Enhancing crop nitrogen efficiency: the role of mixed nitrate and ammonium supply in plant growth and development, Biology, 14, 546, https://doi.org/10.3390/biology14050546, 2025.
Younsi, S. E. and Bouziane, Z.: Plant diversity in Mediterranean coastal dune systems subjected to anthropogenic disturbances, Biodivers. Res. Conserv., 72, 25–38, https://doi.org/10.14746/biorc.2023.72.4, 2023.
Zhang, H., Cui, C., Li, S., Lv, W., Zhang, J., Zhu, X., Xu, C., Wang, Q., Bai, N., and Zhang, H.: Bio-organic fertilizer with Bacillus velezensis promoted plant growth by regulating soil microbial community structure and C/N cycle function, Plants, 15, 382, https://doi.org/10.3390/plants15030382, 2026.
Zhu, W., Gu, S., Jiang, R., Zhang, X., and Hatano, R.: Saline-alkali soil reclamation contributes to soil health improvement in China, Agriculture, 14, 1210, https://doi.org/10.3390/agriculture14081210, 2024.
Short summary
Coastal soils often suffer from salt stress and low fertility, limiting plant growth. We studied beneficial soil bacteria living around the roots of a native coastal plant in Morocco to see how they help plants grow. Several bacteria improved plant height and biomass by making nutrients more available and protecting roots. These findings show that natural soil microbes can support sustainable agriculture and help restore degraded coastal lands.
Coastal soils often suffer from salt stress and low fertility, limiting plant growth. We studied...