Journal cover Journal topic
SOIL An interactive open-access journal of the European Geosciences Union
Journal topic

Journal metrics

Journal metrics

  • IF value: 3.343 IF 3.343
  • IF 5-year value: 4.963 IF 5-year
  • CiteScore value: 9.6 CiteScore
  • SNIP value: 1.637 SNIP 1.637
  • IPP value: 4.28 IPP 4.28
  • SJR value: 1.403 SJR 1.403
  • Scimago H <br class='hide-on-tablet hide-on-mobile'>index value: 25 Scimago H
    index 25
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

  23 Jul 2020

23 Jul 2020

Review status
This preprint is currently under review for the journal SOIL.

Oxygen isotope exchange between water and carbon dioxide in soils is controlled by pH, nitrate availability and microbial biomass through links to carbonic anhydrase activity

Sam P. Jones1,2, Aurore Kaisermann1, Jerome Ogee1, Steven Wohl1, Alexander W. Cheesman3, Lucas A. Cernusak3, and Lisa Wingate1 Sam P. Jones et al.
  • 1INRA, UMR ISPA, 33140, Villenave d’Ornon, France
  • 2Instituto Nacional de Pesquisas da Amazônia, Manaus – AM, CEP 69060-001, Brasil
  • 3College of Science and Engineering, James Cook University, Cairns, Queensland, Australia

Abstract. The oxygen isotope composition (δ18O) of atmospheric carbon dioxide (CO2) can be used to estimate gross primary production at the ecosystem-scale and above. Understanding how and why the rate of oxygen isotope exchange between soil water and CO2 (kiso) varies can help to reduce uncertainty in the retrieval of such estimates. The expression and activity of carbonic anhydrases in soils are important drivers of variations in kiso. Here we estimate kiso and measure associated soil properties in laboratory incubation experiments using 44 soils sampled from sites across western Eurasia and northeastern Australia. Observed kiso exceeded theoretical uncatalysed rates indicating the significant influence of carbonic anhydrases on the variability observed among the soils studied. We identify soil pH as the principal source of variation, with greater kiso under alkaline conditions suggesting that shifts in microbial community composition or intra-extra cellular dissolved inorganic carbon gradients induce the expression of more or higher activity forms of carbonic anhydrases. We also show for the first time in soils that the presence of nitrate under acidic conditions reduces kiso, potentially reflecting the direct or indirect inhibition of carbonic anhydrases. This effect was confirmed by a supplementary ammonium nitrate fertilisation experiment conducted on a subset of the soils. Future changes in atmospheric nitrogen deposition or land-use may thus influence carbonic anhydrase activity. Greater microbial biomass also increased kiso under a given set of chemical conditions likely highlighting the ubiquity of carbonic anhydrase expression by soil microbial communities. These data provide the most extensive analysis of spatial variations in soil kiso to date and indicate key controls required to predict variations in kiso at the scales needed to improve efforts to constrain gross primary productivity using the δ18O of atmospheric CO2.

Sam P. Jones et al.

Interactive discussion

Status: open (until 12 Nov 2020)
Status: open (until 12 Nov 2020)
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
[Subscribe to comment alert] Printer-friendly Version - Printer-friendly version Supplement - Supplement

Sam P. Jones et al.

Sam P. Jones et al.


Total article views: 289 (including HTML, PDF, and XML)
HTML PDF XML Total Supplement BibTeX EndNote
209 66 14 289 13 9 11
  • HTML: 209
  • PDF: 66
  • XML: 14
  • Total: 289
  • Supplement: 13
  • BibTeX: 9
  • EndNote: 11
Views and downloads (calculated since 23 Jul 2020)
Cumulative views and downloads (calculated since 23 Jul 2020)

Viewed (geographical distribution)

Total article views: 246 (including HTML, PDF, and XML) Thereof 246 with geography defined and 0 with unknown origin.
Country # Views %
  • 1



No saved metrics found.


No discussed metrics found.
Latest update: 20 Oct 2020
Publications Copernicus